Fig. 4: lncRNA AC003092.1 enhances TMZ sensitivity through TFPI-2- mediated cell apoptosis.

a Cell viability of U87TR-V-AC and U251TR-V-AC cells after si-NC or si-TFPI-2 treatment measured by CCK-8 assay. ^*P < 0.05 compared with U87TR-V-AC + si-NC cells or U251TR-V-AC + si-NC cells. b The TMZ sensitivity of U87TR-V-AC and U251TR-V-AC cells after intervention of si-NC or si-TFPI-2 determined by CCK-8 assay. ^*P < 0.05 compared with si-NC-treated cells. The mRNA (c) and protein (d, g) level of TFPI-2 in U87TR and U251TR after transfection of V-NC or V-TFPI-2. ^*P < 0.05 compared with V-NC-treated cells. e Cell viability and (f) the TMZ sensitivity of U87TR and U251TR cells after V-NC or V-TFPI-2 transfection. ^*P < 0.05 compared with V-NC-treated cells. h The protein level of proapoptotic proteins (cleaved caspase 3, cleaved caspase 8, cleaved caspase 9, and cleaved PARP) after V-NC or V-TFPI-2 treatment in U87TR and U251TR cells. ^*P < 0.05 compared with V-NC-treated cells. The ratio of apoptosis of U87TR-V-AC and U251TR-V-AC cells after si-NC or si-TFPI-2 transfection with or without 50 μg/ml TMZ treatment for 48 h determined by flow cytometry (i) and TUNEL (j). ^*P < 0.05 compared with si-NC-treated cells. ^&P < 0.05 compared with si-NC +TMZ-treated cells. Scale bar = 50 μm. Data are presented as mean ± SD of three independent experiments