Fig. 6: miR-1249 inhibited CRC growth and angiogenesis by VEGFA, and suppressed migration and invasion by both VEGFA and HMGA2.

a miR-1249-overexpressioning HCT116 and HT29 cells that transfected with blank vector or pcDNA3.1-VEGFA and pcDNA3.1-HMGA2 were subjected to western blot for VEGFA and HMGA2. b, c. VEGFA upregulation could promote proliferation of miR-1249-overexpressing CRC cells using CCK-8 (b) and EdU (c). d, e VEGFA or HMGA2 upregulation could abrogate the effects of miR-1249-overexpressing on migration (d) and invasion (e) of CRC cells. f The ability of HUVECs cultured in TCM from VEGFA-upregulating CRC cells was obviously increased. Results were presented as mean ± SD. All the experiments were performed more than three times. *P < 0.05, **P < 0.01