Fig. 3: PDGF-BB promotes myofibroblast-like differentiation of Smpd1−/− SMCs. | Cell Death & Disease

Fig. 3: PDGF-BB promotes myofibroblast-like differentiation of Smpd1−/− SMCs.

From: Contribution of p62/SQSTM1 to PDGF-BB-induced myofibroblast-like phenotypic transition in vascular smooth muscle cells lacking Smpd1 gene

Fig. 3

SMCs were under control condition or stimulated with PDGF-BB (30 ng/ml) for the indicated time. a Representative western blot showing the protein expression of SM22, α-SMA, and Calponin. b Representative immunoblotting analysis of Vimentin, Collagen I, and FSP-1. c Summarized data of immunoblotting mentioned above. *P < 0.05, **P < 0.01 (n = 4). d Sirius Red staining of SMCs under control condition or stimulated with PDGF-BB (30 ng/ml) for 24 h. e Total collagen amount was examined according to Sirius red staining results. *P < 0.05, **P < 0.01 (n = 4). Two-way ANOVA with genotype and treatment as category factors. f Representative western blot showing the protein expression of TGF-β1 in Smpd1+/+ and Smpd1−/− SMCs before and after PDGF-BB stimulation. The experiment was repeated at least three times with similar results

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