Fig. 8: Akt-p62/SQSTM1 axis contributes to PDGF-BB induced myofibroblast-like phenotype transition of Smpd1−/− SMCs. | Cell Death & Disease

Fig. 8: Akt-p62/SQSTM1 axis contributes to PDGF-BB induced myofibroblast-like phenotype transition of Smpd1−/− SMCs.

From: Contribution of p62/SQSTM1 to PDGF-BB-induced myofibroblast-like phenotypic transition in vascular smooth muscle cells lacking Smpd1 gene

Fig. 8

Smpd1−/− SMCs were infected with p62/SQSTM1 shRNA or scramble shRNA lentiviral particles and then treated with PDGF-BB (30  ng/ml) for 24  h. a Representative immunoblotting and summarized data show the protein expression of p62/SQSTM1 in Smpd1−/− SMCs infected with p62/SQSTM1 shRNA or scramble shRNA (SC) lentiviral particles. *P < 0.05, **P < 0.01 (n = 3). b Real-time RT-PCR analyses of IL-6 and IL-18 mRNA expression in PDGF-BB or vehicle treated Smpd1−/− SMCs with p62/SQSTM1 silencing or not. *P < 0.05, **P < 0.01 (n = 6). c Representative immunoblotting and summarized data show the effects of p62/SQSTM1 shRNA on PDGF-BB-induced protein expression of FSP-1 and TGF-β1 in Smpd1−/− SMCs

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