Fig. 6: ANXA1-suppresed autophagy induces EMT-like alterations in NPC cells. | Cell Death & Disease

Fig. 6: ANXA1-suppresed autophagy induces EMT-like alterations in NPC cells.

From: RETRACTED ARTICLE: Annexin A1-suppressed autophagy promotes nasopharyngeal carcinoma cell invasion and metastasis by PI3K/AKT signaling activation

Fig. 6

a QRT-PCR showing the mRNA expression levels of ANXA1, E-cadherin, N-cadherin, Vimentin, and Snail in the ANXA1 KD 5–8F cells, ANXA1 OE 6–10B cells and their control cells. b Western blot analysis showing the protein expression levels of E-cadherin, N-cadherin, Vimentin and Snail in the ANXA1 KD 5–8F cells, ANXA1 OE 6–10B cells and their control cells. c Western blot analysis showing the protein expression levels of E-cadherin, N-cadherin, Vimentin, and Snail in the EphA2 KD 5–8F cells and scramble shRNA control cells transfected with BECN1 siRNA or ATG5 siRNA (left), or treated with 3-MA (right). d Western blot analysis showing the Snail protein expression level in the ANXA1 KD 5–8F cells and scramble shRNA control cells transfected with SQSTM1 expression plasmid, and ANXA1 OE 6–10B cells and vector control cells transfected with SQSTM1 siRNA. e QRT-PCR showing the Snail mRNA expression level in the ANXA1 KD 5–8F cells and scramble shRNA control cells transfected with SQSTM1 expression plasmid, and ANXA1 OE 6–10B cells and vector control cells transfected with SQSTM1 siRNA. Mean ± SD (n = 3 replicates) and statistical significance are denoted; *P < 0.05; **P < 0.01; ***P < 0.001; no, no significance

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