Fig. 3: Deletion of CREPT leads to significant mitotic cell accumulation. | Cell Death & Disease

Fig. 3: Deletion of CREPT leads to significant mitotic cell accumulation.

From: CREPT/RPRD1B associates with Aurora B to regulate Cyclin B1 expression for accelerating the G2/M transition in gastric cancer

Fig. 3

a–c The mitotic index increases in CREPT deletion cells. Cells were harvested and stained with PI and H3S10p antibody. FACS analyses showed increased percentage of H3Ser10-positive cells when CREPT was deleted (CREPT–/–) (a). A quantitative presentation of the mitotic index from three independent experiments (b) **p < 0.01. A western blot showed the level of H3Ser10 phosphorylation. H3 and β-actin were used as loading controls (c). d Immunofluorescence visualization of mitotic cells. Wild-type (WT) and CREPT deletion (CREPT–/–) MGC803 cells were stained for H3S10p (red) and DAPI (blue). Scale bars, 100 μm. e The percentage of H3S10p-positive cells. Approximately 4000 cells were counted per sample in three independent experiments; **p < 0.01. f Overexpression of HA-CREPT rescues the CREPT deletion-induced mitotic arrest. HA-CREPT was stably induced into CREPT deletion cells. g A quantitative presentation of the mitotic index. Three independent experiments were analyzed by Student's t-test; **p < 0.01, *p < 0.05

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