Fig. 5: The A54970 strain inhibits inflammasome activation and pyroptosis. | Cell Death & Disease

Fig. 5: The A54970 strain inhibits inflammasome activation and pyroptosis.

From: Inhibition of inflammasome activation by a clinical strain of Klebsiella pneumoniae impairs efferocytosis and leads to bacterial dissemination

Fig. 5

a, b BMDMs differentiated from WT were incubated or not with 100 ng.mL−1 of LPS for 3 h and then infected with the A28006 or the A54970 strain (MOI 1:5) for 90 min. Cells were washed twice with a cocktail of antibiotics in PBS, one last wash with PBS. After 18 h, the supernatant was collected. a IL-1β levels were measured by ELISA and b LDH release was measured in the supernatant. c, d BMDMs from WT and Casp1/11−/− mice were incubated with the A28006 or the A54970 strains (MOI 1:5) for 90 min. Cells were incubated for 24 h more. After then, cells were infected with E. coli (MOI 1:20) for 4 h and the supernatant was collected for c TNF-α, IL-12, and IL-1β levels determination by ELISA. d Cell lysis was evaluated by LDH release in the supernatant. a Data represent the mean ± SEM of one independent experiment performed in triplicate. b Data represent one independent experiment. c, d Data represent the mean ± SEM of three independent experiments performed in triplicate. Statistical analysis: one-way ANOVA and Tukey post hoc tests. *P < 0.05

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