Fig. 3: FHC enhances p53 expression through the negative modulation of miR-125b in NSCLC in vitro and ex vivo. | Cell Death & Disease

Fig. 3: FHC enhances p53 expression through the negative modulation of miR-125b in NSCLC in vitro and ex vivo.

From: Ferritin heavy subunit enhances apoptosis of non-small cell lung cancer cells through modulation of miR-125b/p53 axis

Fig. 3

Taqman analysis of miR-125b expression in A549 and H460 cells either transfected with pc3FHC expression vector alone (A549pc3FHC and H460pc3FHC) or co-transfected with pc3FHC/miR-125b mimic (A549pc3FHC/miR-125b mimic and H460pc3FHC/miR-125b mimic) (up). Western Blot assay revealed that protein p53 levels increased in A549pc3FHC and H460pc3FHC vs A549pc3DNA and H460pc3DNA control cells and decreased in A549pc3FHC/miR-125b mimic and H460pc3FHC/miR-125b mimic vs A549pc3FHC and H460pc3FHC (down). Each assay was performed on three independent biological replicates. *P < 0.05 (a, b). Absolute quantitative real-time PCR of miR-125b, p53, and FHC in 22 human NSCLC tissue specimens are reported as Box Plot. Absolute qPCR was performed on three independent technical replicates. Wilcoxon signed-rank test ***P < 0.001 p53 vs miR-125b, Wilcoxon signed-rank test °°°P < 0.001 FHC vs miR-125b (c)

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