Fig. 3: Inhibition of lncRNA-6195 promotes cell proliferation in vitro. | Cell Death & Disease

Fig. 3: Inhibition of lncRNA-6195 promotes cell proliferation in vitro.

From: A novel lncRNA, TCONS_00006195, represses hepatocellular carcinoma progression by inhibiting enzymatic activity of ENO1

Fig. 3

a, b lncRNA-6195 shRNA promoted cell proliferation as assessed by the CCK-8 assay after stable transfection of lncRNA-6195 shRNA(RNAi) or negative control shRNA (NC) in HepG2/6195 (a) and Huh7 cells (b). c, d Inhibition of lncRNA-6195 promotes cell proliferation in HepG2/6195 cells as assessed by the colony formation assay. e, f Western blot results showed that Cyclin D1 protein is upregulated in lncRNA-6195 knock-downed HepG2/6195 cell lines. g, h Inhibition of lncRNA-6195 decreases the cell numbers in the G1 phase and promotes cell cycle progression as analyzed using flow cytometry. (*P < 0.05, **P < 0.01,***P < 0.001, Student’s t test. Data are represented as mean ± SD.)

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