Fig. 3: miR-145-5p enhances Ago2 mRNA translation.
From: Argonaute 2 drives miR-145-5p-dependent gene expression program in breast cancer cells
a, b RIP (RNA Immuno Precipitation) assays performed in control (CTR) and miR-145-5p overexpressing (145-5p) MDA-MB-231 cells using antibodies directed to Ago2, Ago1, or IgG as negative control. mRNA abundance of Ago2 was evaluated by RT-qPCR and normalized over GAPDH mRNA; a representative experiment of a biological duplicate is shown. c Cytoplasmic extracts from MD-MB-231 cells overexpressing miR-145-5p and the relative control cells were loaded on 15–50% sucrose gradients and fractions measured by absorbance at 253 nm (left panel). Fraction density decreases from left to right; a representative profile out of two independent biological replicates is showed. Right panel: pooled fractions of RNA associated with polysomes, monosomes and free RNA were analyzed by RT-qPCR. The percentage of Ago2 mRNA enrichment, normalized over GAPDH mRNA is reported
