Fig. 2: Immunohistochemical staining of glutaminase 1 (GLS1) in human colorectal carcinoma and adjacent normal colorectal tissue. | Cell Death & Disease

Fig. 2: Immunohistochemical staining of glutaminase 1 (GLS1) in human colorectal carcinoma and adjacent normal colorectal tissue.

From: Glutaminase 1 expression in colorectal cancer cells is induced by hypoxia and required for tumor growth, invasion, and metastatic colonization

Fig. 2

a, b Representative images of GLS1 (a) and GLS2 (b) expression in tissue microarrays containing 39 cases of colorectal adenocarcinoma and 15 cases of normal colorectal tissue are shown. The bottom panel shows higher magnification of GLS1 and GLS2 staining (brown). c–e Representative images of GLS1 in tissue microarrays containing tumor samples from 85 colorectal cancer patients (24 patients with the presence of lymph node metastasis) are shown, respectively. c Adjacent normal colorectal tissue; d Primary colorectal tumor tissue; e Lymph node metastasis. The bottom panel shows higher magnification of GLS1 staining. Scale bar = 200 μm; f Image analysis was performed to determine the percentage of GLS1-positive area per field under × 40 magnification based on six random fields per section (n = 24). For bar graph, one-way analysis of variance (ANOVA; mean ± SEM) was used to determine the p-value, ***p < 0.0001

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