Fig. 3: SNAI1 expression affects EndoMT in BECs. | Cell Death & Disease

Fig. 3: SNAI1 expression affects EndoMT in BECs.

From: Inflammation-induced endothelial to mesenchymal transition promotes brain endothelial cell dysfunction and occurs during multiple sclerosis pathophysiology

Fig. 3: SNAI1 expression affects EndoMT in BECs.

a CLDN5, TAGLN, FN1, and FSP1 mRNA expression levels were measured in SNAI1-overexpressing cells by qRT-PCR. Values were normalized using GAPDH and plotted as fold change of control. b, c The TEER (Rb, barrier resistance) and the permeability to FITC-dextran (A.U., arbitrary unit) of the SNAI1-overexpressing cells were measured and plotted as % of control/EV-transduced BECs. d SNAI1 KD was achieved by lentiviral delivery of shRNA. SNAI1, CLDN5, TAGLN, FN1 and FSP1 mRNA expression level in SNAI1 KD cells were measured by qRT-PCR. Values were normalized using GAPDH and plotted as fold change relative to control. Data presented are the mean of triplicate values ± SEM of three independent experiments. Statistical analysis was performed using Student’s t-test or one-way ANOVA where *p < 0.05, **p < 0.01, ***p < 0.001 with post-hoc Bonferroni correction

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