Fig. 1: Identification of EGFR-AS1, which is upregulated in RCC tissues. | Cell Death & Disease

Fig. 1: Identification of EGFR-AS1, which is upregulated in RCC tissues.

From: Long noncoding RNA EGFR-AS1 promotes cell growth and metastasis via affecting HuR mediated mRNA stability of EGFR in renal cancer

Fig. 1

a Flow chart of identification of EGFR-AS1 using GEO datasets and TCGA. b EGFR-AS1 expression between RCC samples and paired normal tissues was compared using qRT-PCR analysis (n = 40). P < 0.001 by the Mann–Whitney U test. c The full-length sequence of EGFR-AS1 was determined using 5′ and 3′ RACE assays. d Schematic annotation of the EGFR-AS1 genomic locus on chromosome 7: 55,179,750-55,188,934 reverse strand and composed of two exons in humans. Blue rectangles represent exons. e EGFR-AS1 identified in the subcellular fraction of 786-O cells using cellular fractionation assays. β-Actin and U6 are cytoplasmic and nuclear markers, respectively. f Representative images of RNA FISH analysis of EGFR-AS1 (green) in RCC tissues. Scale bar = 50 μm. g FISH analysis of EGFR-AS1 in 786-O and KETR-3 cells using a biotin-labeled RNA probe. Nuclei were stained with DAPI. Scale bar = 50 μm

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