Fig. 1: Upregulated ZEB1-AS1 in pulmonary fibrosis is positively correlated with ZEB1 expression. | Cell Death & Disease

Fig. 1: Upregulated ZEB1-AS1 in pulmonary fibrosis is positively correlated with ZEB1 expression.

From: lncRNA ZEB1-AS1 promotes pulmonary fibrosis through ZEB1-mediated epithelial–mesenchymal transition by competitively binding miR-141-3p

Fig. 1

RT-qPCR was carried out to determine the relative expression of ZEB1-AS1 (a) and ZEB1 (b) mRNA in BLM-induced lung tissues (IPF group, n = 10) and normal lung tissues (Normal group, n = 10). Spearman analysis was used to analyze the association between ZEB1-AS1 and ZEB1 expression in the lung tissues from Normal group (c) and IPF group (d). Relative expression of ZEB1-AS1 (e) and ZEB1 (f) mRNA in RLE-6TN cells treated with 10 ng/ml TGF-β1 for 48 h, as detected by RT-qPCR. g RNA FISH was performed to determine the location of endogenous ZEB1-AS1 (red) in RLE-6TN cells. U6 and GAPDH were used as nuclear and cytoplasmic localization markers, respectively. DNA (blue) was stained with DAPI. h Nucleocytoplasmic separation result confirmed that ZEB1-AS1 was mainly expressed in the cytoplasm by using RT-qPCR. *P < 0.05, **P < 0.01

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