Fig. 5: Linc-GALH regulates the Gankyrin gene methylation level in both HCC tissues and cells. | Cell Death & Disease

Fig. 5: Linc-GALH regulates the Gankyrin gene methylation level in both HCC tissues and cells.

From: The long non-coding RNA Linc-GALH promotes hepatocellular carcinoma metastasis via epigenetically regulating Gankyrin

Fig. 5: Linc-GALH regulates the Gankyrin gene methylation level in both HCC tissues and cells.

a The bisulfite sequencing method was applied to examine the methylation rate of the Gankyrin promoter in normal liver tissues, cirrhosis liver tissues, primary HCC tumor tissues and the tumor tissues of HCC patients with PVTT. Representative results of each group were shown and the difference of each group was analyzed. b Representative bisulfite sequencing analysis of the Gankyrin promoter in HCC cells transduced with Linc-GALH shRNA or Linc-GALH Lv, respectively. c, d Relative mRNA and protein levels of Gankyrin in HCC cells upon Linc-GALH alteration were detected by qPCR and western blot assays, respectively. e The protein level of Gankyrin was evaluated in SMMC-7721 cells treated with 5-aza-dC (DAC) and DMSO as control. Each experiment was performed in triplicate independently; **P < 0.01. P < 0.05 was regarded as statistically significant

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