Fig. 6: Linc-GALH induces EMT and mainly mediated by Gankyrin in HCC.
a IHC assessment of intensity of Gankyrin, E-cadherin and N-cadherin in 10 Linc-GALH-High and 10 Linc-GALH Low sections from HCC patients (original magnification × 200). A strong positive correlation of Linc-GALH expression with N-cadherin levels (r2 = 0.4997, P = 0.0005) and a strong negative correlation of Linc-GALH expression with E-cadherin levels (r2 = 0.5066, P = 0.0004) was gained. b Representative pictures of immunochemical staining of lung sections for Gankyrin, E-cadherin and N-cadherin in the Linc-GALH knockdown and controlled SMMC-7721 cells and Linc-GALH overexpressed and controlled HepG2 cells (original magnification × 200). c Wound healing assays was performed to examine the in vitro migration abilities of Linc-GALH overexpressed HepG2 Huh7 cells transduced with shGankyrin (original magnification × 40). d The in vitro invasion abilities of Linc-GALH overexpressed HepG2 and Huh7 cells transduced with shGankyrin were investigated using transwell assays (original magnification × 100). Each experiment was performed in triplicate independently; **P < 0.01. P < 0.05 was regarded as statistically significant
