Fig. 7: Linc-GALH regulates the ubiquitination of DNMT1 and involves the regulation of AKT signaling. | Cell Death & Disease

Fig. 7: Linc-GALH regulates the ubiquitination of DNMT1 and involves the regulation of AKT signaling.

From: The long non-coding RNA Linc-GALH promotes hepatocellular carcinoma metastasis via epigenetically regulating Gankyrin

Fig. 7: Linc-GALH regulates the ubiquitination of DNMT1 and involves the regulation of AKT signaling.

a Expression levels of DNMT1, DNMT3A and DNMT3B were detected by western blot in HCC cells when Linc-GALH was knockdown or upregulated. b Expression of DNMT1 was confirmed by western blot in HepG2 cells treated with cycloheximide (100 μg/mL). c HepG2 cells transfected with Linc-GALH Lv or the control lentivirus were cultured with or without MG132, and western blot was conducted using anti-DNMT1 antibody. d The lysates of HCC cells transduced with Linc-GALH shRNA or Linc-GALH Lv lentivirus and their control group were conducted to immunoprecipitated with anti-DNMT1 antibodies to detect polyubiuitination of DNMT1 with anti-Ub antibodies. e The expression levels of EMT related genes were examined by western blot in HCC cells upon Linc-GALH alteration. f p-AKT was evaluated by immunoblot analysis with AKT protein and β-actin as controls

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