Fig. 5: Lic and Hep act in parallel in the JNK pathway.

Fluorescent micrographs of third instar wing discs (a–l) are shown. Compared with the GFP-expressing control (a), RNAi-mediated depletion of hep, dTAK1 or wnd failed to suppress ptc > Lic induced cell invasion (b–d). ptc-Gal4 driven expression of Hep^WT induced a large number of cells migrating to the posterior of wing disc, which was not suppressed by expressing GFP (e) or lic-RNAi (f), but was synergistically enhanced by co-expressing Lic (g) or MKK3 (h). ptc > Egr induced cell migration (i) was partially inhibited in hep (j) or lic (k) heterozygous mutants, and fully blocked in their transheterozygous mutants (l). Scale bars, 20 μm. Statistics of migrated cell numbers was shown in (m–o), and One-way ANOVA test was used to calculate statistical significance, n ≥ 18, mean + s.d., n.s., P > 0.05; ***P < 0.001