Fig. 1: Dpp blocks autophagy and midgut degradation.
From: Crosstalk between Dpp and Tor signaling coordinates autophagy-dependent midgut degradation
a Expression of Dpp and TkvACT prevents midgut removal. Histology from control (NP1-GAL4/+), Dpp (NP1-GAL4/+; UAS-dpp/+) and TkvACT (NP1-GAL4/+; UAS-tkvACT/+) midguts from late third instar animals (−4 h RPF) shows enlarged midgut and gastric caeca (arrows). Scale bar represents 200 μm. b Autophagy flux visualized using GFP-mCherry-Atg8a in midgut cells from control (NP1-GAL4/UAS-GFP-mCherry-Atg8a), Atg1RNAi (NP1-GAL4/UAS-GFP-mCherry-Atg8a; UAS-Atg1RNAi/+), and Dpp (NP1-GAL4/UAS-GFP-mCherry-Atg8a; UAS-dpp/+) larvae at −4 h RPF. Scale bar represents 20 μm. c Expression of dominant-negative TOR (TorTED) suppresses the phenotype of of Dpp and TkvACT. Morphology from TorTED (NP1-GAL4/UAS-TorTED) with the co-expression of Dpp (NP1-GAL4/UAS-TorTED; UAS-dpp/+) and TkvACT (NP1-GAL4/UAS-TorTED; UAS-tkvACT/+) midguts from −4 h RPF shows contracted gastric caeca (arrows). Scale bar represents 200 μm. d Quantification of gastric caeca size (data represent as the average pixels ± SD; **p < 0.0001)
