Fig. 2: Genetic interactions between Dpp signaling and Tor.
From: Crosstalk between Dpp and Tor signaling coordinates autophagy-dependent midgut degradation
Knockdown of Tor suppresses the phenotype of Dpp and TkvACT. a Morphology from TorRNAi knockdown line #2 (NP1-GAL4/+; UAS-Tor RNAi#2/+) with the expression of Dpp (NP1-GAL4/+; UAS-dpp/UAS-TorRNAi#2) and TkvACT (NP1-GAL4/+; UAS-TorRNAi#2/UAS-TkvACT) midguts from −4 h RPF shows contracted gastric caeca (arrows). Scale bar represents 200 μm. b Quantification of gastric caeca size (data represent as the average pixels ± SD; **p < 0.0001). c Morphology from a second independent TorRNAi knockdown line (NP1-GAL4/+; UAS-Tor RNAi/+) with the expression of Dpp (NP1-GAL4/+; UAS-dpp/UAS-TorRNAi) and TkvACT (NP1-GAL4/+; UAS-TorRNAi/UAS-TkvACT) midguts from −4 h RPF shows contracted gastric caeca (arrows). Scale bar represents 200 μm. d Quantification of gastric caeca size (data represent as the average pixels ± SD; **p < 0.0001). e Histology sections from +12 h RPF show the knockdown of TorRNAi with TkvACT expression promotes animal survival with midgut contraction similar to controls (circled). f Number of animals that pupated from combined TorRNAi knockdown with either expression of Dpp or TkvACT
