Fig. 1: Loss-of-function of Pif1A results in male-sterile. | Cell Death & Disease

Fig. 1: Loss-of-function of Pif1A results in male-sterile.

From: Drosophila Pif1A is essential for spermatogenesis and is the homolog of human CCDC157, a gene associated with idiopathic NOA

Fig. 1

a Whole-mount in situ hybridization for control probe (left) and Pif1A probe (right) on testis of adult flies. b Schematic representation of the Pif1A locus. Pif1A is located on chromosome 3R at 85B1–85B1 on the cytogenetic map. Filled tetragonums indicate exons; the straight lines between filled tetragonums indicate introns. Pif1A1 is a frame shift mutant allele with 2 bp deletion. c Real-time PCR analysis of Pif1A transcriptional levels of wild-type animals, Pif1A1 mutants, Pif1A1 over deficiency (Pif1A1/DF) animals and the duplication of Pif1A genomic DNA in the background of Pif1A1 (Pif1A-GFP.FPTB/Cyo;Pif1A1/Pif1A1). d Male fertility test. y-axis represented embryo hatch rate. The male fertility was zero in Pif1A1 mutants and Pif1A1/DF animals and partially restored in the duplication of Pif1A genomic DNA in the background of Pif1A1 animals

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