Fig. 1: Colorectal and breast cancer cell lines escape chemotherapy-mediated senescence.
From: Regulation of senescence escape by TSP1 and CD47 following chemotherapy treatment
a Following sn38 treatment (5 ng/ml), senescence was detected by the evaluation of p21waf1 expression, SA-β-galactosidase, PML bodies, and ɣ-H2AX staining (n = 3 +/−sd, Mann–Whitney test, *** = p < 0.001). b After treatment, LS174T cells were washed and stimulated with 10% FBS for 7 days to reinduce cell growth. Persistent MCF7 cells (PMC) were generated using the same protocol following doxorubicin treatment. c Images illustrating persistence using colorectal (top) or breast (bottom) cells. SA-β-galactosidase activity staining shows PLC heterogeneity and the presence of proliferating cells (white cells, named PLD) together with senescent cells (blue cells, named PLS). d In vivo evaluation of tumor formation by parental LS174T cells, senescent cells, or PLCs. Senescent and emergent cells were generated as described above. Cells were injected subcutaneously in NOD/SCID mice (five mice were used per condition in each experiment, two-way Anova with a Bonferroni’s multiple comparison test: ns = p > 0.05. ** = p < 0.01). e Quantification of necrosis in tumors arising from parental or PLC cells (n = 6, Mann–Whitney test, * = p < 0.05, see also supplementary Figure 1)
