Fig. 3: METTL3 regulates STAT3/KLF4/SOX2 pathway by mediating the expression of JAK2 and SOCS3. | Cell Death & Disease

Fig. 3: METTL3 regulates STAT3/KLF4/SOX2 pathway by mediating the expression of JAK2 and SOCS3.

From: m6A methylation controls pluripotency of porcine induced pluripotent stem cells by targeting SOCS3/JAK2/STAT3 pathway in a YTHDF1/YTHDF2-orchestrated manner

Fig. 3

a qPCR analysis of JAK2 and SOCS3 in control and METTL3 knockdown piPSCs. GAPDH was used as an internal control. b Western blot analysis of JAK2 and SOCS3 in piPSCs with or without METTL3 knockdown. β-Actin was used as loading control. c Western blot analysis of JAK2 and SOCS3 in piPSCs with or without METTL3 overexpression. d Western blot analysis of JAK2, pSTAT3, STAT3, KLF4, and SOX2 in piPSCs with or without METTL3 overexpression and transfected with negative control or JAK2 siRNA. e Western blot of nuclear and cytoplasmic distribution of pSTAT3, KLF4, and SOX2 in piPSCs with or without METTL3 overexpression and transfected with negative control or JAK2 siRNA. f qPCR analysis of SOX2 and KLF4 expression in piPSCs with or without METTL3 overexpression and transfected with negative control or JAK2 siRNA. g Western blot analysis of SOCS3, pSTAT3, STAT3, KLF4, and SOX2 in piPSCs with or without METTL3 knockdown and transfected with negative control or SOCS3 siRNA. h Western blot of nuclear and cytoplasmic distribution of pSTAT3, KLF4, and SOX2 in piPSCs with or without METTL3 knockdown and transfected with negative control or SOCS3 siRNA. Histone H3 and Tubulin serve as nuclear and cytoplasmic markers, respectively. i qPCR analysis of SOX2 and KLF4 expression in piPSCs with or without METTL3 knockdown and transfected with negative control or SOCS3 siRNA. Data were presented as mean ± SD of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 compared with the control group

Back to article page