Fig. 6: MTA2 enhances the potential of migration and invasion, and activates the PI3K/AKT signaling in PDAC cells in a PTEN-dependent manner.
From: MTA2-mediated inhibition of PTEN leads to pancreatic ductal adenocarcinoma carcinogenicity
a, b MIA Paca-2 or PANC-1 cells were infected with shSCR, shMTA2, or shMTA2 plus shPTEN, respectively. a Cell migration or b invasion was determined using a transwell migration or invasion assay, respectively. Representative images (magnification, ×20) are shown. Data are presented as mean ± S.D. for at least three independent experiments. *P < 0.05. c Western blot analysis was used for investigating endogenous phosphorylation levels of the PI3K and AKT following MTA2 inhibition in MIA Paca-2 or PANC-1 cells, and the additional introduction of MTA2 was used for the rescued assay. d Western blot analysis was used for investigating endogenous phosphorylation levels of the PI3K and AKT in PTEN-knockdown MIA Paca-2 or PANC-1 cells following MTA2 inhibition
