Fig. 6: RhoA/ROCK is responsible for the spinal nerve ligation (SNL)-induced phosphorylation of p38 MAPK and hyperalgesia. | Cell Death & Disease

Fig. 6: RhoA/ROCK is responsible for the spinal nerve ligation (SNL)-induced phosphorylation of p38 MAPK and hyperalgesia.

From: P2Y12 regulates microglia activation and excitatory synaptic transmission in spinal lamina II neurons during neuropathic pain in rodents

Fig. 6

Immunoblots of GTP-RhoA (a) and ROCK2 (b) from total cell lysates of ipsilateral spinal cord tissue. Densitometric analysis of GTP-RhoA (c) and ROCK2 (d) were normalized to the respective loading controls. The expression of GTP-RhoA and ROCK2 increased after SNL at days 3, 7, and 14 after surgery. Immunoblots of ROCK2 (e) and p-p38 (f) from total cell lysates of ipsilateral spinal cord tissue 7 days after SNL surgery. Densitometric analysis of ROCK2 (g) and p-p38 (h) were normalized to the respective loading controls. g The P2Y12 antagonist, MRS2395 partially suppressed the increased ROCK2 expression after nerve ligation. h The ROCK inhibitor, Y27632, reversed the increased phosphorylation of p38 MAPK induced by SNL. i, j Y27632 inhibited the mechanical allodynia (i) and thermal hyperalgesia (j) on the ipsilateral side after SNL surgery. Y27632 was intrathecally injected three times a day, 3 μg per injection per rat, beginning at 1 day before surgery and continuing for 6 days. Y27632 reversed the mechanical allodynia for up to 7 days after surgery. Injury-induced thermal hyperalgesia was also suppressed by Y27632 from day 1 to 5. Y27632 alone had no effect on the mechanical allodynia and thermal hyperalgesia. The data are presented as the mean ± SEM. n = 6 rats per group. *p < 0.05, vs. sham group, **p < 0.01, vs. sham group, ***p < 0.001, vs. sham group; #p < 0.05, vs. SNL group, ###p < 0.001, vs. SNL group

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