Fig. 3: MiR-185 silencing promotes the osteogenic function of mesenchymal stem cells (MSCs). | Cell Death & Disease

Fig. 3: MiR-185 silencing promotes the osteogenic function of mesenchymal stem cells (MSCs).

From: Mmu-miR-185 depletion promotes osteogenic differentiation and suppresses bone loss in osteoporosis through the Bgn-mediated BMP/Smad pathway

Fig. 3

MSCs were derived from the bone marrow of wild-type (WT) or knockout (KO) mice, and induced with osteoblast induction medium (OIM) for osteogenic differentiation. a Alkaline phosphatase (ALP) staining in WT or KO MSCs after osteoblast induction for 7 days. Scale bar = 500 μm. b ALP activity determination in MSCs after osteogenic induction for 7 days. c Alizarin Red S Staining in MSCs after osteoblast induction for 21 days. Scale bar = 500 μm. d Matrix mineralization was quantified in MSCs after induction for 21 days. e Real-time PCR showed the messenger RNA (mRNA) expression levels of osterix (Osx), collagen type 1α 1 (Col1a1), and osteocalcin (OC) in MSCs after induction with OIM for 7 days. f The expression of osteoblast marker genes in MSCs cultured for 3 days in OIM was indicated by western blot. Data were shown as mean ± S.D (*P < 0.05, **P < 0.01, ***P < 0.001)

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