Fig. 4: Depletion of miR-185 attenuates bone loss in ovariectomized (OVX) mouse model. | Cell Death & Disease

Fig. 4: Depletion of miR-185 attenuates bone loss in ovariectomized (OVX) mouse model.

From: Mmu-miR-185 depletion promotes osteogenic differentiation and suppresses bone loss in osteoporosis through the Bgn-mediated BMP/Smad pathway

Fig. 4

Femur samples were harvested 6 weeks after OVX or Sham operation. a The bone mineral density (BMD) values of mice femurs were calculated by micro-computed tomography (μCT) (n = 5). b Three-dimensional (3D) reconstructive images of trabecular bones in femur epiphysis. Scale bar = 400 μm. c Real-time PCR indicated the miR-185 expressions in femurs of wild-type (WT) Sham and WT OVX groups. d The bone volume/total volume (BV/TV), trabecular bone surface/bone volume (BSA/BV), trabecular bone thickness (Tb. Th), trabecular bone number (Tb. N), trabecular bone space (Tb. Sp), and trabecular pattern factor (Tb. Pf) of femur epiphysis were calculated by μCT (n = 5). e Bone samples were decalcied and bone slices were prepared. Representative images of hematoxylin and eosin (H&E), Masson’s trichrome staining and Fast green staining were shown. Scale bar = 200 μm. f The relative area of trabecular bone and collagen were analyzed using Image J program (n = 3). g The femur bone tissue (removed of bone marrow) were grinded with liquid nitrogen, and the RNA was extracted. mRNA expression of osteoblast markers in OVX groups was determined by real-time PCR. Data were shown as mean ± S.D (*P < 0.05, **P < 0.01, ***P < 0.001)

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