Fig. 1: The expression of CXCR7 in metastatic melanoma cells and clinical samples.

a The expression levels of chemokine receptors in B16-F10 cells were evaluated by qRT-PCR and normalized to Ccr1 mRNA level. b, c The relative mRNA (b) and protein (c) levels of CXCR7 in B16-F0, B16-F1, and B16-F10 cells. The mRNA levels were normalized to B16-F0 cells. d Representative images of CXCR7 expression in benign, malignant, and metastatic melanoma samples that illustrate scores of 0, 1, 2, and 3. The top images were taken at ×100 original magnification (scale bar = 200 μm) and the bottom images were taken at ×200 original magnification (scale bar = 100 μm). e The correlation of CXCR7 staining scores with tumor stages. The χ2 test was used to assess the correlation between categorical variables. f Overall survival of melanoma patients with high (n = 24) or low (n = 78) CXCR7 expression. The expression cutoff = 3.51 FPKM. Overall survival was analyzed by Kaplan–Meier survival analysis and the log-rank test. The qRT-PCR experiments were independently repeated three times. Data are presented as mean ± SD; *p < 0.05, **p < 0.01 compared with B16-F0 and B16-F1 cells