Fig. 3: Sortilin promotes the EMT-like mesenchymal transition of glioblastoma. | Cell Death & Disease

Fig. 3: Sortilin promotes the EMT-like mesenchymal transition of glioblastoma.

From: Sortilin promotes glioblastoma invasion and mesenchymal transition through GSK-3β/β-catenin/twist pathway

Fig. 3

a, b Western blotting analysis of the expression levels of classical mesenchymal markers (N-cadherin, MMP-9, and vimentin) and T-cadherin, U87, and A172 cell lines that were treated with 400 nM AF38469 or Si-Sor. c, d Representative immunofluorescence images of phalloidin (green) and nucleus (blue) in U87 and A172 cell lines that were treated with 400 nM AF38469; scale bar = 50 μm. e, f Representative cellular morphology change of U87 and A172 cell when transfected with Si-Sor. Scale bar = 10 μm. g The numbers of filopodia were calculated from five random image of each group and shown in histogram. Error bars (SD) represent the data of triplicate samples for each group. Paired two-way Student’s t-test; *p < 0.05, **p < 0.01

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