Fig. 7: AF38469 inhibits glioblastoma invasion and mesenchymal transition in vivo.

a The gross observation of subcutaneous glioblastoma at day 21 post injection (Upper) and the volume of tumor were measured and calculated every week after transplanting. Scale bar = 10 mm (*p < 0.05, **p < 0.01). b Western blotting analysis of the activation of GSK-3β/β-catenin/twist and the expression levels of EMT markers (N-cadherin, MMP-9, vimentin, and T-cadherin) in subcutaneous glioblastoma tissues. Error bars (SD), paired two-way Student’s t-test, *p < 0.05, **p < 0.01. c Representative MRI images (scale bar = 2 mm) and gross observations (scale bar = 1 mm) of orthotopic glioblastoma xenografts in the indicated groups. d The volumes of orthotopic xenografts were detected by MRI and calculated every week after transplanting. e H&E staining to show the margin of orthotopic xenografts, dotted black line to show the border of glioblastoma. Scale bar = 50 μm. f The survival time of glioblastoma-bearing mice was analyzed by Kaplan–Meier method and compared by log-rank test. *p < 0.05, **p < 0.01