Fig. 6: Effects of pharmacological inhibition of TFEB on LC3 relocation and cell death induced by SQ1 and SQ2.
From: Squaramide-based synthetic chloride transporters activate TFEB but block autophagic flux
a Experimental design to assess LC3 puncta when the synthetic chloride transporters SQs were combined with different inhibitors. b Representative images of U2OS GFP-LC3 expressing cells when pretreated with the calcium chelator BAPTA-AM, the antioxydant NAC, the calcineurin inhibitor CsA, its analogue CsH, and the protein synthesis inhibitor CHX followed by the co-treatment with the synthetic chloride modulators. c Bars show the average number of GFP-LC3+ dots per cell. Data are expressed as means ± SD of one representative experiment *P < 0.05; **P < 0.01; ***P < 0.001. d Evaluation of cell death by double-staining with DAPI together with DiOC6(3) followed by cytofluorometric detection of dead cells (DAPI+) and dying cells (DAPI+ DiOC6(3)low). Data are expressed as means ± SEM of at least three independent experiments (Co/Ctrl). *P < 0.05; **P < 0.01; ***P < 0.001
