Fig. 4: Oxidized ATM promotes intercellular citrate accumulation by upregulating PFKP and CS in hypoxic TNBC cells. | Cell Death & Disease

Fig. 4: Oxidized ATM promotes intercellular citrate accumulation by upregulating PFKP and CS in hypoxic TNBC cells.

From: RETRACTED ARTICLE: Intracellular citrate accumulation by oxidized ATM-mediated metabolism reprogramming via PFKP and CS enhances hypoxic breast cancer cell invasion and metastasis

Fig. 4

a Intracellular citrate of BT549 and Hs578T treated with or without hypoxia (1% O2, 12 h) were measured by LA Assay Kit. b Intercellular citrate was measured by LA Assay Kit with indicated culture. c PFKP and CS expression of BT549 and Hs578T treated with or without hypoxia were determined by western blotting. d PFKP and CS expression of BT549 (left) and Hs578T (right) with indicated treatments were tested by western blotting. e Intracellular citrate was determined in hypoxic BT549 and Hs578T with shRNA against shPFKP, shCS, and their control cells. f Ectopic PFKP or CS was transfected into ATM-knocked down, hypoxic BT549 and Hs578T cells, and intracellular citrate were measured. g PFKP and CS proteins in normal and different grade breast tumor tissues were detected with IHC staining. The quantitative diagram of PFKP or CS expression were shown. h Citrate levels in breast tumor tissues and normal tissues were detected. i Bioinformatics analysis of PFKP (left) and CS (right) in breast cancer patients from METABRIC database (*P < 0.05; **P < 0.01)

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