Fig. 9: Enhanced effects of oxidized ATM on metastasis of TNBC in vivo.

a Representative images of metastases in mice lung examined by H&E staining (scale bar, 20 μm and b the quantitative diagrams of metastases in mice lung. c Citrate contents in mice tumors. The citrate content was normalized by quality of tissue. d–f Expressions of p-ATM, PFKP, and CS of mouse tumor tissues were detected by western blotting (d) and IHC (e) (scale bar, 20 μm). f The quantitative diagram of e were shown. g–i Expressions of MMP2 and MMP9 in mouse tumor tissues were detected by western blotting (g) and IHC (h) (scale bar, 20 μm). i The quantitative diagram of h were shown. j A schematic diagram depicts the role of oxidized ATM promoting TNBC cell invasion and metastasis via EMR-mediated of citrate accumulation to activate AKT/ERK/MMP2/9 signaling