Fig. 8: Pharmacologic induction of autophagy exerts a protective effect against NF-κB activation and cytokines production. | Cell Death & Disease

Fig. 8: Pharmacologic induction of autophagy exerts a protective effect against NF-κB activation and cytokines production.

From: ATG5-mediated autophagy suppresses NF-κB signaling to limit epithelial inflammatory response to kidney injury

Fig. 8: Pharmacologic induction of autophagy exerts a protective effect against NF-κB activation and cytokines production.The alternative text for this image may have been generated using AI.

a Immunoblot analyses of indicated proteins in the kidneys at day 7 after sham and UUO injury treated with or without rapamycin. b Immunoblot analyses of indicated proteins in the kidneys at day 7 after sham and UUO injury treated with or without 3-MA. c HK-2 cells were pretreated with vehicle or rapamycin prior to Ang II stimulation. Nuclear or cytoplasmic fractions were analyzed by immunoblotting with p-p65, p-65, fibrillarin (nuclear marker), and β-actin (cytoplasmic marker) antibodies. d HK-2 cells were pretreated with vehicle or 3-MA prior to Ang II stimulation. The amount of p-65 and p-p65 in the nuclear and cytoplasmic extracts was examined by immunoblot analysis. e The mRNA levels of IL-1β, IL-6 and TNF-α in HK-2 cells pretreated with vehicle or 3-MA followed by Ang II stimulation. Data are means ± SEM (n = 3). *P < 0.001 vs. Ang II-untreated cells; #P < 0.05 vs. Ang II-treated cells without 3-MA exposure. f Immunoblot analyses of indicated proteins among groups

Back to article page