Fig. 2: TIAM1 promotes chemoresistance in CRC cells.
From: TIAM1 promotes chemoresistance and tumor invasiveness in colorectal cancer
a The bar graphs illustrate mRNA expression of TIAM1 in CRC cell lines transfected by siRNA against TIAM1 or the corresponding control evaluated by qRT-PCR. b Western blot analyses show protein expression of TIAM1 in CRC cell lines transfected by siRNA against TIAM1 or the corresponding control. The relative expression values normalized to reference protein are shown in each image. c CRC cell lines transfected by TIAM1 siRNA or the corresponding control were incubated for 48 h with the indicated concentrations of 5-FU, L-OHP, and CPT-11 and then assayed for cell viability; the line graphs are presented as the treated/control cell ratio and the bar graphs illustrate IC50. d The invasion ability of CRC cell lines transfected by TIAM1 siRNA or the corresponding control was quantified using a trans-well invasion assay. Representative microscopic fields are shown (left) and the data is presented as the treated/control cell ratio (right). Scale bars, 200 μm (×100). Error bars are presented as mean ± SE of three independent experiments, *P < 0.05; **P < 0.01; ***P < 0.001 (T-test)
