Fig. 5: CAFs regulate chemoresistance through the upregulation of TIAM1 expression.
From: TIAM1 promotes chemoresistance and tumor invasiveness in colorectal cancer
a Preparation of CM derived from CAFs. b The bar graphs illustrate the mRNA expression of TIAM1 in CRC cell lines co-cultured with CAF-derived CM established from two different CRC patients for 24 or 48 h. c The western blotting analyses illustrate the protein expression of TIAM1 in CRC cell lines co-cultured with CAF-derived CM established from two different CRC patients for 48 h. The relative expression values normalized to reference protein are shown in each image. d CRC cell lines transfected by TIAM1 siRNA or the corresponding control were co-cultured with CAF-derived CM or the corresponding control for 48 h with the indicated concentrations of 5-FU, then assayed for cell viability; the line graphs are presented as the treated/control cell ratio and the bar graphs illustrated IC50. e CRC cell lines co-cultured with CM derived from CAFs, transfected by TIAM1 siRNA or the corresponding control were incubated for 48 h with the indicated concentrations of 5-FU, and then assayed for cell viability; the line graphs are presented as the treated/control cell ratio. f Bar graphs illustrate mRNA expression of NANOG, OCT-4, SOX2, and ALDH in CRC cell lines co-cultured with CAF-derived CM or corresponding control for 48 h. g Bar graphs illustrate mRNA expression of Nanog, Oct-4, Sox2, and ALDH in CRC cell lines co-cultured with CAF-derived CM or the corresponding control for 48 h. Error bars are presented as mean ± SE of three independent experiments, *P < 0.05; **P < 0.01; ***P < 0.001 (T-test)
