Fig. 4: PD-L2 knockdown suppresses epithelial–mesenchymal transition and inactivates RhoA-ROCK-LIMK2 signaling. | Cell Death & Disease

Fig. 4: PD-L2 knockdown suppresses epithelial–mesenchymal transition and inactivates RhoA-ROCK-LIMK2 signaling.

From: Osteosarcoma cell intrinsic PD-L2 signals promote invasion and metastasis via the RhoA-ROCK-LIMK2 and autophagy pathways

Fig. 4

a EMT markers and MMP9 were measured by western blotting in KHOS and U2OS cells after transfection. b PD-L2 knockdown reduced p-LIMK and p-cofilin expression. c Expression of BMPR2, RGMb, and NEO1 in osteosarcoma cell lines detected by western blotting. d PD-L2 knockdown reduced RGMb and NEO1 expression. e PD-L2 knockdown decreased BMPR2 expression (top), but BMPR2 silencing and overexpression did not affect PD-L2 expression (bottom). f Bioinformatics prediction indicated there may be co-expression between PD-L2 and RhoA (http://genemania.org/). g GTPase assay verified RhoA changes in U2OS cells. h The expression levels of BBRN supercomplex (RGMb, BMPR2, and Neo1) in the primary and metastatic osteosarcoma tumors were evaluated by IHC (magnification × 200)

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