Fig. 1: Ectopic BAG3 expression induces autophagy.
From: BAG3 promotes autophagy and glutaminolysis via stabilizing glutaminase
a HepG2 or MCF7 cells were infected with lentivirus containing empty or BAG3 construct. Total protein was extracted and protein expression of indicated autophagy-related genes were investigated by immunoblotting. b HepG2 or MCF7 cells infected with lentivirus containing empty or BAG3 construct were treated with vehicle, CQ and E64D plus pepstatin A respectively, protein expression levels of LC3, p62 and BAG3 were analyzed using Western blot analysis. c HepG2 or MCF7 cells stably overexpressing EGFP-LC3B were infected with lentivirus containing empty or BAG3 construct. Cells were treated with vehicle, CQ or E64D plus pepstatin A, and the punctate distribution of EGFP-LC3B was visualized under the florescence microscopy. d Quantitative analysis of (c). Results shown represent mean ± SD from five representative microscopic fields and three independent experiments were performed. e HepG2 or MCF7 cells transduced with lentivirus containing empty or BAG3 construct, and ultrastructure was analyzed using transmission electron microscopy. *P < 0.05. Error bars indicate means ± SD
