Fig. 3: BAG3 promotes autophagy via upregulation of GLS.

a Western blot analysis of changes induced by BAG3 overexpression in GLS, LC3 conversion and p62 levels in indicated cells. b–e HepG2 or MCF7 cells were infected with lentivirus containing empty or BAG3 construct, and glutamine consumption (b), intracellular glutamate production (c), intracellular α-KG (d), and ammonia production (e) were analyzed using the colorimetric method. f Control or BAG3-overexpressing HepG2 cells were transfected with scramble shRNA or shRNA specific against GLS (shGLS), and Western blot analysis was performed using the indicated antibodies. g and h Control or BAG3-overexpressing HepG2 cells were transfected with scramble shRNA or shRNA specific against GLS (shGLS), and glutamine consumption (g), ammonia production (h) were analyzed using the colorimetric method. i Control or BAG3-overexpressing MCF7 cells were transfected with scramble shRNA or shRNA specific against GLS (shGLS), and Western blot analysis was performed using the indicated antibodies. *P < 0.05. N.S. not significant. Error bars indicate means ± SD