Fig. 4 | Cell Death & Disease

Fig. 4

From: BAG3 promotes autophagy and glutaminolysis via stabilizing glutaminase

Fig. 4

BAG3 stabilizes GLS by suppression its proteasomal degradation. a HepG2 or MCF7 cells were infected with lentivirus containing empty or BAG3 construct, and GLS mRNA expression was measured using RT-qPCR. b The effect of overexpressing BAG3 on the half-life of GLS was evaluated in indicated cells treated with cyclohexamide (CHX). c Control or BAG3-overexpressing HepG2 and MCF7 cells were treated with vehicle, MG132 or E-64d plus pepstatin A respectively, GLS protein expression was investigated using Western blotting. *P < 0.05. N.S. not significant. Error bars indicate means ± SD

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