Fig. 6: Lysine-specific demethylase 1 (LSD1) promotes the release of proinflammatory mediators in hepatitis B virus (HBV)-infected HK-2 cells via toll-like receptor 4 (TLR4). | Cell Death & Disease

Fig. 6: Lysine-specific demethylase 1 (LSD1) promotes the release of proinflammatory mediators in hepatitis B virus (HBV)-infected HK-2 cells via toll-like receptor 4 (TLR4).

From: The histone demethylase LSD1 promotes renal inflammation by mediating TLR4 signaling in hepatitis B virus-associated glomerulonephritis

Fig. 6

a Western blot analysis of TLR4 in HK-2 cells co-transfected with pCMV-HBV1.3 and shCtrl or shTLR4 for 48 h. b Enzyme-linked immunosorbent assay (ELISA) analysis of proinflammatory mediator levels in culture medium of HK-2 cells co-transfected with pCMV-HBV1.3 and shTLR4-2 for 48 h. c ELISA analysis of proinflammatory mediators levels in culture medium of HK-2 cells infected by HBV treated with shLSD1-2 along with pcDNA3.1/myc or pcDNA3.1/myc-TLR4 for 48 h. d, e Western blot analysis of p-IKKα/β, IKKα, IKKβ, p-IκBα, IκBα (d), p-JNK1/2, T-JNK1/2, p-ERK1/2, T-ERK1/2, p-p38MAPK, and T-p38MAPK (e) in HK-2 cells co-transfected with pCMV-HBV1.3 and shLSD1-2 and pcDNA3.1/myc-TLR4 for 12 h. f, g ELISA analysis of proinflammatory mediators levels in cell culture medium. HK-2 cells were pre-incubated with or without 30 µm PDTC (f) or 20 µm SP600125 (g) for 2 h and then treated with pCMV-HBV1.3 along with pcDNA3.1/myc or pcDNA3.1/myc-LSD1 for 48 h. Data are presented as the mean ± SD (N = 4). *P < 0.05 versus shCtrl; #P < 0.05 versus shLSD1-2 + vector group; †P < 0.05 versus HBV + shLSD1-2 group; §P < 0.05 versus no PDTC or SP600125 treatment

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