Fig. 5: Fmr1 is a direct target of miR-129-5p.
From: MicroRNA-129 modulates neuronal migration by targeting Fmr1 in the developing mouse cortex
a A schematic diagram showing a miR-129-5p binding site in the Fmr1 3′UTR. The mutated seed sequence is in red. b Luciferase reporter containing wild type or mutated 3′UTR of Fmr1 was co-transfected with miR-129-5p, miR-129-3p or control mimics and the normalised luciferase activity was determined. The results are expressed as the mean ± SD. Comparisons were performed by Student’s t-test, and the statistically significant P-values are shown as *P < 0.05, **P < 0.01 and ***P < 0.001. c FMRP protein levels in miR-129-5p, miR-129-3p or negative control (NC) mimics-transfected N1E-115 cells were detected by western blot analysis using a specific FMRP antibody. β-actin was used as the loading control
