Fig. 1
From: Bone marrow-derived Ly6C− macrophages promote ischemia-induced chronic kidney disease
CCR2 deficiency alleviates acute kidney injury, but aggravates renal fibrosis after I/R. a Scheme: time-related comparison after I/R for AKI and CKD. b, c Representative kidneys from CCR2–/– and WT mice and quantitative analyses for the area of medullary congestion (b) and kidney weight after I/R (c). d Representative photomicrographs of Periodic Acid-Schiff staining after I/R injury. Graphs indicate the tubular damage and infiltrating leukocytes after I/R. e, f Representative photomicrographs (left) of collagen staining by Masson blue (e) and Sirius red (f) at day 15 after I/R in the WT and CCR2–/– kidneys. Corresponding graphs (right) indicate percentage of Masson blue and Sirius red. g Western blots of α-SMA and PDGFR-β at day 15 after I/R in the WT and CCR2–/– kidneys. Quantitation of α-SMA and PDGFR-β relative to GAPDH. Scale bars, 100 μm. N = 3–6/group. *P < 0.05, **P < 0.01, ***P < 0.001. Values were means±SD
