Fig. 3

Ly6C⁻macrophages, which are the dominant macrophages in WT kidneys in CKD after I/R injury, are mostly CX3CR1⁺, derived from bone marrow and incapable of differentiating into α-SMA⁺cells. a The percentage (left) and number (right) of Ly6C⁻ and Ly6C⁺ macrophage analyses by flow cytometry from whole kidneys in WT mice. b The analyses by flow cytometry of percentage of CX3CR1⁺ and CX3CR1⁻ macrophages in WT kidneys at d7 after I/R gated on F4/80⁺Ly6C⁻. Graphs and representative plots. c Scheme of bone marrow chimera. d The percentage of GFP⁻ and GFP⁺ cells among Ly6C⁻ macrophage analyses by flow cytometry from the peripheral blood (left) and kidneys (right) of bone marrow chimeric mice at d10/d20 after I/R. Graphs and representative plots. e The analyses by flow cytometry of the percentage (middle) and number (right) of α-SMA⁺ and α-SMA⁻ cells among Ly6C⁻GFP⁺ macrophages from kidneys of bone marrow chimeric mice at d10/20 after I/R. Graphs and representative plots. Scale bars,100 μm. N = 4–6/group. n.s. P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001. Values were means±SD. Mø, macrophages. BMC, bone marrow chimera