Fig. 6
From: Bone marrow-derived Ly6C− macrophages promote ischemia-induced chronic kidney disease
Adoptive transfer of renal Ly6C−macrophages into immune-deficient kidneys directly induces renal fibrosis. a Scheme. Ly6C− macrophages from I/R kidneys (d5) were injected to NCG mice under renal capsule. The mice were killed at day 5 after injection. Macrophages extracted from sham group as control. b–d Representative photomicrographs and graphs for Periodic Acid-Schiff staining (b), Sirius Red staining (c) and Masson Blue staining (d). e Levels of mRNA encoding α-SMA, Fibronectin, Collagen I by Q-PCR. f Representative photomicrographs and graphs for immunofluorescence labeled F4/80 (red). g Representative images and double positive analyses for immunofluorescence labeled α-SMA (green) and F4/80 (red). h Scheme. Ly6C− macrophages from CAG-EGFP I/R kidneys (d5) were injected to NCG mice under renal capsule. The mice were killed on day 5 after injection. i Representative images and double-positive analyses for immunofluorescence labeled α-SMA (red) and GFP (green). Scale bars, 100 μm. N = 3/group. *P < 0.05, ***P < 0.001. Values were means ± SD. Mø, macrophages. NCG, NOD-Prkdcem26Cd52Il2rgem26Cd22/Nju. Mø, macrophages
