Fig. 3: CTBP1 modulates the expression of cell adhesion genes and favors a mesenchymal phenotype.

Gene expression was assessed by RT-qPCR at different times, after seeding PC3 cells on a collagen matrix. a Expression levels of several cell adhesion genes were analyzed by RT-qPCR on total and adherent populations. Data were normalized to GAPDH and control. b ChIP-qPCR using CTBP1 or nonspecific (Gal4) antibodies and primers located at −0.11 kb upstream of the TSS of ITGB4 or −0.2 kb upstream of the HBB TSS promoter region