Fig. 4: Analysis of the cell membrane portion involved in attachment to substrate.

a Confocal microscopy images of rhodamine–phalloidin stained PC3 cells with differential CTBP1 expression levels. b Cellular dimensions, width and length, and the number of +cells (cells with a high count of filopodia) was estimated as cells with three or more filopodia sets. Cell dimensions were normalized to PC3.PGIPZ cells.PC3.PGIPZ, n = 23; PC3shCTBP1, n = 43; PC3.pcDNA3, n = 25; PC3.CTBP1, n = 35. Data were analyzed by one-way ANOVA. *p ≤ 0.05