Fig. 5: PCa and MeS spontaneous metastasis model. | Cell Death & Disease

Fig. 5: PCa and MeS spontaneous metastasis model.

From: CTBP1 depletion on prostate tumors deregulates miRNA/mRNA expression and impairs cancer progression in metabolic syndrome mice

Fig. 5

Four-week-old NSG mice were fed a CD or HFD for the entirety of the experiment. a Body weight was measured once a week (left) and on the 12th week of diet mice were inoculated in the left flank with PC3.PGIPZ (control) or PC3.shCTBP1 (CTBP1 depleted) cells. Tumor size was measured three times a week (right). b CTBP1 and CTBP2 mRNA levels at the time of sample collection was assessed by RT-qPCR on tumor samples. c Lung metastasis burden was inferred by the mRNA levels of human GAPDH, detected in RNA samples from mice lung tissue. d H&E (left) and CTBP1 IHC (inmunohistochemical) staining (right) from lungs of mice bearing xenografts, arrows indicate metastatic cells. e Representative images of CTCs harvested from PC3.PGIPZ (n = 1) or PC3.shCtBP1 (n = 3) inoculated NSG mice, 1 week after blood extraction. Bars represent average and SD of two independent experiments. Data were analyzed by two-way ANOVA. *p ≤ 0.05

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