Fig. 1: MiR-155 is delivered by AAV-9 to the pancreas. | Cell Death & Disease

Fig. 1: MiR-155 is delivered by AAV-9 to the pancreas.

From: Inhibition of miR-155 reduces impaired autophagy and improves prognosis in an experimental pancreatitis mouse model

Fig. 1

a Schematic representation of AAV-9 used in this study with the locations of the miRNA and GFP indicated. b Real-time PCR analysis of miR-155 levels after 3 weeks of transfection with AAV-9 harboring miR-155 or miR-155 sponge (n = 6 per group, *P < 0.05, **P < 0.01, ***P < 0.001). c Fluorescence microscopy showing efficient transduction of pancreas, as indicated by GFP expression, 3 weeks following AAV administration (×200). d Sequence alignment of TAB2 with the seed sequence of miR-155 using bioinformatics algorithms. The target prediction program identified a potential miR-155 binding site in TAB2. e–g Western blot and IF results showing that TAB2 expression is regulated by miR-155 (n = 3 per group, *P < 0.05, **P < 0.01)

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