Fig. 5: Expression of RIP1, RIP3 and MLKL were increased and the complex formation between TRAF2 and MLKL was increased in the ischemic striatum following MCAO.

a Representative images of the western blot analysis of RIP1, RIP3 and MLKL in the striatum of sham-operated or MCAO mice at 24 h after reperfusion. Contra: contralateral side. Ipsil: ipsilateral side. b Quantification data showing that RIP1, RIP3 and MLKL were significantly increased in the ischemic striatum at 24 h after MCAO (n = 3). N.S.: not significant; *p < 0.05; **p < 0.01; ***p < 0.001. c Total proteins in the lysates of striatum were incubated with anti-MLKL antibody and protein A/G agarose beads, and TRAF2 and MLKL levels in the lysates (input) and in the immunoprecipitated proteins (IP) were analyzed by western blot. Representative images were shown. d Quantification data of the protein levels of immunoprecipitated TRAF2 and MLKL, showing that the complex formation between TRAF2 and MLKL was increased in the ischemic striatum. The IP quantification data were also normalized to the total protein levels of TRAF2 and MLKL, respectively (n = 3). N.S.: not significant; *p < 0.05; **p < 0.01